مشخصات پژوهش

In this study, a practical approach has been developed to detect ovarian specific antigen (CA-125) with high sensitivity and specificity. The method utilizes enhanced differential pulse voltammogram signals of a selfassembled prussian blue nanoparticles (PB) decorated on MXene Quantum dots (QDs) supported by electrodeposited Au nanoparticles modified glassy carbon electrode (AuNP/PB/MXene QD/GCE). The fabrication procedure was completed by casting an optimized value of streptavidin and CA-125 antibody on the transducing platform. The improved electrochemical characteristics of the MXene QD/PB/AuNPs modification were ascribed to the combined impacts of MXene QD, PB, and AuNPs. The integration of MXene QD-PB composite within the modified layer contributed to enhanced mechanical strength. Additionally, the inclusion of gold nanoparticles (AuNPs) notably enhanced conductivity and promoted the attachment of anti-CA-125 onto the modified glassy carbon electrode (GCE), consequently improving sensitivity. Several analytical methods, such as energydispersive X-ray spectroscopy (EDS) and field emission scanning electron microscopy (FE-SEM) were utilized to investigate the structural, elemental composition and morphological characteristics. The functionality of the newly created immunosensor was evaluated using electrochemical impedance spectroscopy, differential pulse voltammetry and cyclic voltammetry to evaluate the synergistic effects. By employing this highly efficient analysis strategy, the measurement of CA-125 with differential pulse voltammetry (DPV) results a remarkable sensitivity with a detection limit of 0.57 pU.mL− 1 and a wide linear range of 1 pU.mL− 1 to 0.12 nU.mL− 1 (R2 = 0.9824). Comparable results were obtained by analyzing serum samples from ovarian cancer patients at various stages. This study offers a significant insight into the advancement of immunosensor techniques for fabricating electrochemical transducers for the clinical detection of CA-125.