مشخصات پژوهش

Plant growth-promoting bacteria (PGPB) are beneficial soil bacteria which stimulate plant growth by various mechanisms. Both feeding of growing world population and environmental pollution are major problems that our world is facing today. So identification and addition of PGPB to plants could provide benefits of increasing agricultural productivity and lessening the use of chemical fertilizer. The association of some Herbaspirillum species with economically important crops in the Poaceae family such as wheat and rice have been proven. In this study, the presence of some important enzymes contributing to plant-beneficial functions was investigated in Herbaspirillum rhizosphaerae proteom. So the sequence of 1-carboxylate (ACC) deaminase, nitrogenase subunit, Indol-3-glyserol phosphate synthase, nitrilase, tryptophan transaminase, indolepyruvate ferredoxin oxidoreductase, indole-3-acetamide hydrolase, indole-3-acetonitrile dehydrogenase, were retrieved from Uniprot protein database. Selected sequences were subjected to BLASTp against non redundant protein sequence (nr) database with Herbaspirillum rhizosphaerae as a selected organism in NCBI server. The results revealed the absence of nitrogenase and presence of the ethylene levels modulating enzyme, ACC deaminase, and some enzymes related to the biosynthesis different pathways of indoleacetic acid (IAA) from tryptophan. The presence of mentioned enzymes may induct plant growth- promoting ability of H. rhizosphaerae