مشخصات پژوهش

RRECTED CA125is aMucinglycoprotein andits concentration in humanserumcorrelates withawoman'sriskofde- veloping ovarian cancerandalsoindicatesresponseto therapyin diagnosedpatients. Accurate detection of this large, complexprotein in patient biofluids is ofgreatclinicalrelevance.In this work,aninnovative im- munoassay for quantitation ofCA125basedonsignalamplification strategy wasproposed.In this work, Horseradishperoxidase(HRP)labeledCA125-antibody(anti-CA125)wasimmobilized ontoagreenandbio- compatiblenanocompositecontainingpolycetyltrimethyl ammoniumbromide(P(CTAB) asconductivema- trix, chitosan(CS)asbiocompatibleagentandslivernanoparticles(Ag NPs)assignalamplification element. Therefore,anovelnanocomposite film basedP(CTAB-CS)andAgNPswasexploitedto developahighly sensitive immunosensorfordetection ofCA125protein. Importantly, AgNPspreparedbyelectrodeposition methodwhichlead to compactmorphology.Fullyelectrochemicalmethodologywasusedto prepareanew transduceronaglassycarbonsurfacewhichprovidedahighsurfaceareato immobilize ahighamountofthe anti-CA125.Thesurfacemorphologyofelectrode wascharacterized byhigh-resolution field emissionscan- ningelectronmicroscope(FE-SEM)andenergydispersivespectroscopy (EDS). Theimmunosensorwasem- ployedforthe detection ofCA125usingcyclicvoltammetry (CV)anddifferentialpulsevoltammetry (DPVs) techniques.Underoptimizedcondition the calibration curveforCA125concentration bySWVandDPVwas linearin 0.01–400U/mLwithlower limit ofquantification of0.001U/mL.Themethodwassuccessfullyap- pliedassayofthe CA125in unprocessedhumanplasmasamples.