مشخصات پژوهش

A simple, sensitive and accessible while reliable method was developed and validated for quantitation of bupreno (Bup) as a highly lipophilic drug in human urine samples. The proposed method is based on a rapid and easy dis micro solid-phase extraction procedure using magnetic molecularly imprinted polymer nanoparticles (MMIPNPs), ma (Fe 3 O 4 ) cores surrounded by polyamidoamine and Bup as template, followed by high-performance liquid chromato analysis with fuorescence detector (HPLC-FL). The prepared MMIPNP adsorbent was characterized by diferent tech Transmission electron microscopy images show that the Fe 3 O 4 core nanoparticles are well enwrapped by MIP la determination process, Bup could be quantitatively extracted using MMIPNPs and then can be easily desorbed by m of sodium hydroxide (0.1 mol/L) and methanol (1:9, v/v) solution before injection to HPLC. The relative recoveries were found to be 97.4–100.3%, and the linear dynamic range was within the ranges of 1–1000 ng/mL with R 2 of Remarkably high quality of 0.21 and 0.71 ng/mL was obtained as the limit of detection and limit of quantifcation, tively. The developed method was successfully applied for determination of Bup in infected human urine samples.