مشخصات پژوهش

The hydrophilic, conductng, biocompatble and porous scaffolds were designed using poly(2-hydroxy ethyl methacrylate)-co-poly(N-isopropylacrylamide)-co-poly(ε-caprolactone) (P(HEMA-b-NIPAAm-bCL))/polyaniline (PANI) for the osteoblast applicatons. To this end, the PHEMA and P(HEMA-b-NIPAAm) were synthesized via reversible additon of fragmentaton chain transfer (RAFT) polymerizaton, and in next step, the ε-caprolactone was polymerized from –OH group of PHEMA segments through the ring opening polymerizaton (ROP). The electroactvity, mechanical propertes, and hydrophilicity of designed scaffolds played an important role in the adhesion, differentaton, and proliferaton of MG63 cells. By using the PHEMA and PNIPAAm, the hydrophilicity and biocompatbility, and by employing the PCL, the appropriate mechanical propertes were acquired. The additon of PANI in the compositon induced the conductvity to scaffolds. The morphology, electrical conductvity, biocompatbility, hydrophilicity and mechanical characteristcs of the nanofbers were thoroughly investgated. The scaffolds possessed a porous nanostructure (nanofber diameter ranged in 60–130 nm) with a large surface area, electrical conductvity of 0.03 S cm–1 and contact angle of 49 ± 5 ͦ , which imitated the natural microenvironment of extra cellular matrix (ECM) to regulate the cell atachment, proliferaton and differentaton. In vitro cytocompatbility studies were performed over 168 h and indicated that the nanofbers were non-toxic to MG63 cells and potent to the artfcial nanostructured osteoblastng.