مشخصات پژوهش

The human immunodeficiency virus type-1 (HIV-1), a member of retrovirus family, has been a causative organism in an acquired immunodeficiency syndrome (AIDS). One of the important enzymes necessary for the replication of this virus is HIV-1 protease (HIV-1 PR). Thus, searching for HIV-1 PR inhibitors from natural sources has become a promising approach [1] . HIV-1 aspartyl protease (PR) plays a key role in virion morphogenesis, underscoring the effectiveness of protease inhibitors (PI) [2] . In the present study, we studied two aptamers with nucleotid sequencing of CCGGGTCGTCCCCTACGGGGACCTAAAGACTGTGTCCAACCGCCCTCGCCT named as AP1 and AP2 with nucleotid sequencing of CTTCATTGTAACTTCTCATAATTTCCCGAGGCTTTTACTTTCGGGGTCCT and a mutant structure of it named as AP3. In the mutated aptamer C, T, A, C and C nucleotids of AP2 were substituted with A, G, G, A and T to yield AP3. The considered aptamers have been studied experimentaly by Duclair et al [2] from the stability and HIV-1 protease binding ability aspects. Structures of HIV-1 protease enzyme was taken from Protein Data Bank (http://www.pdb.org/pdb/). Classical molecular dynamics simulations were performed using NAMD package [3] , and visual molecular dynamics (VMD) [4] was used for visualization and analysis of the trajectories. The results of the simulation including structural results like RMSD, hydrogen bonds and radial distribution functions as well as energetics of the complex like interaction energy and the contribution of van der Waals and electrostatic interactions in it have been extracted and discussed. The structural results show that the aptamers bind to the active site of considered enzyme and the energetic results show that the aptamers have relatively high affinity to their targets. A further study reveals that there is a direct correlation between the length of RNA aptamer and the strength of aptamer binding to the target. In addition to the above results, we have also described the nature of interactio