مشخصات پژوهش

The need to develop innovative and reformative approaches for the design of chemical sensors has increased in recent years. It is because of demands for selectivity, stability, and reproducibility. In this respect, nano-mimetic enzymes with their unique features can provide an efficient and convenient platform for chemical sensors. Cholesterol is a construction unit of hormonal system in mammals and also an important component of cell membrane. It plays a vital role in the synthesis of several vitamins, steroid hormones and bile acids. Therefore, the concentration of cholesterol is monitored most often in food and clinical samples [1]. In this study, a simple and one-pot method is developed for the preparation of Zr-based metal-organic framework (MOF) which provides a large surface area and active sites [2]. The obtained MOF exhibits intrinsic peroxidase-like catalytic activity. With the Zr-based MOF as a catalyst, the peroxidase substrate of terephthalic acid (TA) can be oxidized by H2O2 to produce the high fluorescent hydroxyl terephthalate (HTA), which provides a unique strategy for the fluorometric detection of H2O2 and cholesterol. With such a process, H2O2 could be detected with a linear range of 0.1 to 100 μM. Meanwhile, using cholesterol oxidase, sensitive detection of cholesterol is also achieved with a linear range of 0.5 to 200 μM and a detection limit of 0.19 μM. A novel TA/Zr-based MOF system was successfully applied for H2O2 and cholesterol determination in real samples. The unique nature of the MOF makes it promising material for the fabrication of low-cost and high-performance biosensors.