مشخصات پژوهش

The stmuli-responsive nanocomposites were designed as drug delivery nanocarriers. Thanks to promising propertes such as large surface area and easy chemical functonalizaton, the graphene derivatves can be used for the drug delivery applicatons. For this purpose, in the current work, the poly(L,D-lactde)- block-poly(N-isopropylacrylamide-rand-acrylic acid) grafed from reduced graphene oxide (rGO-grafPDLA-block-P(NIPAAm-rand-AAc)) was synthesized by the ring opening polymerizaton (ROP) and atom transfer radical polymerizaton (ATRP). As compared with the traditonal radical polymerizatons, living polymerizatons are among the most-utlized methods to achieve surface initated polymer brushes as they provide excellent control over the polymers compositon. The average sizes of rGO-graf-PDLA-blockP(NIPAAm-rand-AAc) nanocomposite using the dynamic light scatering (DLS) measurements at pH values of 4.0 and 7.4 were 240 and 150 nm, respectvely. The lower critcal soluton temperature (LCST) of rGOgraf-PDLA-block-P(NIPAAm-rand-AAc) was determined to be 39 °C through the ultraviolet-visible (UVVis) spectroscopy. The doxorubicin hydrochloride (DOX)-loading capacity was 99 %. The release of DOX increased at 42 °C compared to 37 °C. The results confrmed that the pH- and temperature-dependent releasing of this drug nano-carrier was benefcial for the antcancer at the tumor-like environment. The biocompatbility was also confrmed by assessing the survival rate of breast cancer cell line (MCF7) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The synthesized nanopartcles would have an excellent potental in the antcancer drug delivery.