مشخصات پژوهش

Organophosphate (OP) toxins, as the most familiar agents of neurotoxins, are widely used in public health, agriculture or by animal husbandry [1]. Global depletion of OPs into the environment threatens public health through widespread pollution of food and water [2]. An excess amounts of diazinon can lead to the dimness of vision, nausea, muscle tremors and, difficult breathing [3]. The application of new diagnostic systems to measure organophosphates has been an important issue for researchers. In this research, a sensitive analytical method provided for indirect determination of diazinon based on the peroxidase-like property of molybdenum disulfide/zirconium-based metal-organic framework nanocomposite (MoS2 @ MIP-202 (Zr)). The inhibitory effect of diazinon on the enzymatic activity of acetylcholinesterase was used to obtain appropriate selectivity. In addition, MoS2 @MIP-202 (Zr) nanocomposite, has a very effective peroxidase-like activity, and significantly increases the very poor emission resulting from the chemiluminescence reaction of the NaHCO3-H2O2 system. In the presence of diazinon and its binding to acetylcholinesterase, the catalysis of acetylcholine to choline decreases and consequently, decreases the concentration of H2O2. So, the emission intensity of the chemiluminescence decreases linearly. The proposed system has a high sensitivity in diazinon measurement. The synthesized MoS2@ MIP-202 (Zr) catalyst could be created a very effective diagnostic system using the green, cost-effective and easy method as well as the easy-to-use chemiluminescence system. A wide linear range of 0.2‒200 ppb and the detection limit of 0.04 ppb obtained for the determination of diazinon using the method. The high repeatability of the method was confirmed by its application for environmental samples.