مشخصات پژوهش

Prostate cancer is the most commonly diagnosed cancer among men after the lung cancer, therefore finding the ways to cure and control of these disease is very important [1]. Triptorelin acetate is the long acting agonist that has the FDA approval in curing prostate cancer. This drug effect on pituitary gland and results in an initial increase in circulating levels of gonadotropins. Continuous administration of triptorelin acetate cause pituitary desensitization and down-regulation, leading to suppressed circulating levels of gonadotropins and sex hormones [2]. Therefore, development of rapid and reliable selective or multicomponent methods is necessary for extraction and detection of this drug in different matrices. Zeolite nano-composite have found a wide range of applications in different sample preparation technologies because of their unique properties [3]. This dissertation aims to develop simple, rapid, sensitive and environment-friendly magnetic solid phase extraction (MSPE) methods based on zeolites as adsorbents. In this work, a magnetic zeolite was synthesized, characterized and then applied as an effective adsorbent for MSPE of triptorelin prior to HPLC analysis. Various experimental parameters affecting the extraction recovery including the amounts of sorbent, volume of desorption solvent, extraction and desorption time, pH, temperature, washing solvent type were optimized. The method was then validated by figures of merit under the optimal conditions. The applicability of the “MSPE” was evaluated by detecting of triptorelin in biological samples.