مشخصات پژوهش

Methamphetamine (MAMP), one of the recreational industrial opiates with sever damage, is commonly abused to create a feeling of extreme pleasure in youth and teenagers. Taking high doses of this famous street opiate leads to the increased heart rate and blood pressure, respiratory problems, stroke and death [1, 2]. To control the epidemic problems of MAMP abuse, the developing a reliable method for the selective and on-site screening of biofluids and seized ecstasy drugs is essential for the legal and medical authorities. Herein, a glassy carbon electrode (GCE) modified with nickel oxide nanoparticles (NiONPs) anchored on MXene has been applied as a supporting substrate to load more aptamer sequences as the MAMP receptors. The GCE modified with NiONPs/MXene nanocomposite as the aptasensor exhibited more surface area and higher current than the bare GCE. The fabricated aptasensor detected MAMP electrochemically for 40 min with a linear concentration range of 1 pM to 50 mM and a limit of detection (LOD) of 0.02 pM. The proposed system indicated a desirable selectivity in the presence of other possible interferences such as cocaine (COC), methadone (MET), tramadol (TRM), pethidine (PTD) and codeine (COD). The aptasensor detected MAMP in some human serum, urine and saliva samples, with the acceptable recoveries ranging from 98.51%-105.94%, 96.80%-100.75% and 97.07%-103.68%, respectively. According to the results the proposed strategy is promising for the fabrication of other electrochemical aptasensors to monitor other opiates to open a new way of dealing with the global problem of addiction.