مشخصات پژوهش

The recent development of microextraction techniques based on deep eutectic solvents (DESs) holds great promise in developing green analytical chemistry. The use of chromatographic instruments in drug analysis is a powerful analytical approach, but it is not practical for on-site analysis in many cases and needs sophisticated users. In this paper, we present a spectrophotometer-based ultrasound-assisted emulsification–microextraction with a hydrophobic deep eutectic solvent for preconcentration and quick determination of concentrations of the anticancer drug, doxorubicin. The spectrophotometer uses 96-well Corning microplates with a microliter volume that is entirely compatible with the microextraction technique. The DES was prepared by mixing choline chloride and phenyl ethanol in a mole ratio of 1:4. The effect of different parameters (pH, DES volume, salt concentration, and sonication time) was optimized by a multivariate approach, central composite design, combined with desirability function. The optimized method showed excellent ability for extraction of doxorubicin from urine samples. The method showed a wide linear range (0.3–15 μg/mL) with a low limit