مشخصات پژوهش

Chickpea (Cicer arietinum L.) is one of the most important staple food legume crops worldwide. For investigating molecular marker development and genetic diversity, expressed sequence tags-simple sequence repeats (EST-SSRs) are of particular importance. However, the number of key genes and molecular markers are limited to undertake molecular breeding in chickpea and deal with abiotic stresses. After downloading the EST sequences from the NCBI website, we determined the contigs and singletons by EGassembler software and the SSR in the EST sequences by SSRLocator software. Therefore, 993 contigs and 1953 singletons were obtained under salinity stress. Accordingly, 16 SSR markers could be introduced for selection of tolerant cultivars to salt stress in chickpea. Moreover, contig294, contig307, and contig982; and singletons including GR403187.1, GR408755.1, and GR403067.1 could be suggested as new genes in controlling the salt stress tolerance mechanism containing SSR markers. The highest percentage of TF in contigs and singletons was associated with AP2, ERF, bZIP, Dof and WRKY. Moreover, the hub genes detected contained SSR marker related to ABC transporter E family and 1-aminocyclopropane-1- carboxylate oxidase. To evaluate the valid of this research, for eight of the contig, specific primers were designed and the result was confirmed by five of them via PCR. Chickpea ESTs generated sets are considered as high-quality transcripts for functional markers development and gene discovery related to abiotic stress tolerance which are able to facilitate chickpea breeding.