مشخصات پژوهش

Electrospun nanofibers from biomaterials have been widely used in regenerative medicine, including tissue engineering applications. Nanofiber matrices have become attractive due to their large surface area, high porosity, controlled mechanical properties, and their ability to interact with cells in a manner which mimics the natural ECMs [1]. Within this class of biomaterials, alginate, a polysaccharide derived from brown algae, exhibits excellent biocompatibility that bears structural resemblance to glycosaminoglycan, one of the major components of ECMs in human tissue. But the utilization of electrospun scaffolds of alginate is strongly limited by its high solubility in aqueous environments and by the difficulty to adjust its degradation dynamics. To overcome these drawbacks, modification of alginate nanofibers with other biopolymers and inorganic materials were developed [2, 3]. Composite nanofibers scaffolds based on alginate with chitosan, gelatin and poly (vinyl alcohol) incorporated with inorganic materials such as hydroxyapatite and montmorillonite were prepared via electrospinning method with different ratio of polymers and fillers. The scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), mechanical measurement and cell-scaffold interaction study. The results exhibited improvement in stability, degradation rate, mechanical properties and cell-scaffold behaviors of composite scaffolds compared to pure electrospun alginate which is useful in tissue engineering (TE) applications. This developing field aims to regenerate damaged tissues by combining cells from the body with porous biomaterials, which act as templates, to guide the growth of new tissues.