مشخصات پژوهش

Enzymes are biological catalysts that are believed to be the cornerstones of life. The combined enzyme in nanoformats is called nanobiocatalyst. Polymeric nanofibers, carbon nanotubes [1], carbon nanosheets, nanocrystals and nanoparticles, can be mentioned, including nanostructures used for enzyme immobilization. Coumarin and its derivatives are important compounds due to their presence in naturally occurring aromatic products found in plants and cinnamon flavoured foods [2]. These compounds have applications including antibacterial, anti-inflammatory, anti oxidant, anticoagulant, anticancer and anti-HIV [3]. Coumarins can be synthesized by various methods such as Pechmann, Perkin, Knoevenagel, Reformatsky and Witting reactions [4]. In this research, we initially synthesized Fe3O4@SiO2 and then aminated, eventually, immobilized the trypsin onto it. Then we used this nanobiocatalyst to synthesize the coumarin derivatives from phenols 1 and β-ketoester 2 via Pechmann condensation in water and ethanol solvents (Fig. 1). The structure of the coumarins obtained is determined by 13CNMR, 1HNMR and IR spectroscopic data.