مشخصات پژوهش

In vitro culture techniques provide useful tools for rapid propagation and improved plant species production. Sium sisarum, commonly known as skirret, belongs to the Apiaceae plant family and is resistant to cold and diseases. This plant has many medicinal properties and is used in many cases, including maintaining bone and cardiovascular health, and strengthening the immune system. Due to the limited geographical distribution of this plant and its improper exploitation, the need to preserve and propagate it with novel techniques such as tissue culture is felt more than before. Due to having deep physiological dormancy and abundant surface contamination, disinfection and germination of skirret seeds is of great importance for creating in vitro cultures. In this study, in order to achieve a suitable method for surface disinfection and seed germination under in vitro conditions, different methods of disinfection and germination conditions and seedling growth was evaluated in a completely randomized factorial design. The results showed that the best disinfection treatment was the combined use of 8% sodium hypochlorite for 15 minutes and 70% ethanol for 1 minute. Finally, the seeds were sown in 1/2MS medium after washing three times with sterile distilled water.