مشخصات پژوهش

Camelina sativa is an emerging low-resource oilseed crop that has significant potential for oil production. It is imperative to modify camelina oils for idealized fatty acid structure that can match diverse application needs. Camelina seed harbors considerable quantities of very long-chain fatty acids (VLCFAs) that may not be advantageous. These VLCFAs can be effi- ciently diminished by disabling the fatty acid elongase1 (FAE1) in camelina. The allohexaploid camelina possesses three alleles of FAE1 genes. Using CRISPR-Cas 9 technology, three alleles of FAE1 genes can be knocked out simultaneously. In this study, pFGC-pcoCas 9 recombinant expression vector containing gRNA designed for FAE1 gene silencing was used. In this research, firstly, somatic embryogenesis improvement and micropropagation of terminal shoot cultures were established from germinated seeds under in vitro conditions and used as target tissues for pFGC-pcoCas 9 recombinant expression vector containing gRNA designed for FAE1 silencing. The constructed recombinant vector was conveyed to the camelina plant by Agrobacterium tumefaciens. Three explants (hypocotyl, cotyledon, and terminal leaves) were utilized with five distinct combinations of plant growth regulators (NAA, BAP, 2,4-D, TDZ, and Kin) to improve somatic embryogenesis and regenera- tion in Camelina. The greatest rate of somatic embryogenesis was observed in the cotyledon explants cultivated in the MS medium including 0.5 mg/L NAA + 5 mg/L BAP, and the maximum frequency of regeneration was recorded in the terminal leaf explant cultured in the MS medium containing 0.5 mg/L NAA + 2 mg/L BAP + 1 mg/L Kin. Transgenic C. sativa plants created by Agrobacterium transformation were confirmed by PCR technique. The genetic transformation efficiency reached 93.62%, indicating a high success rate in gene editing. This investigation illustrated that the CRISPR/Cas 9 system can be effectively applied to the polyploid crop camelina to quickly acquire featur