مشخصات پژوهش

In this study, n-octanol loaded polyvinylidene fluoride (PVDF) membrane sheet was introduced as extracting phase in high-throughput liquid phase thin film microextraction (TFME) for the rapid and simultaneous extraction of two steroid hormones, including estrone (E1) and 17β-estradiol (E2) in aqueous samples (water, waste water and urine samples) prior to liquid chromatographic determination. The proposed thin film membrane was characterized by FE-SEM, AFM, ATR and BET. Thin film membrane was prepared by non-solvent induced phase inversion method and pore generation using Polyvinylpyrrolidone (PVP). Optimum extraction/desorption conditions were found as: organic solvent: n-Octanol, Salt concentration: 16%w/v NaCl, stirring speed: 1600 rpm, pH 8, extraction temperature: 25 °C, extraction time: 35 min, desorption solvent: methanol and desorption time: 15 min. The figures of merit were obtained under the optimized conditions: the Linear Dynamic Ranges (LDRs): 6.70-100 μg L-1 and 4.18-100 μg L-1, Limits of Detection (LODs): 2.21 μg L-1 and 1.38 μg L-1, Limits of Quantification (LOQs): 6.70 μg L-1 and 4.18 μg L-1 for E1 and E2, respectively.