مشخصات پژوهش

A magnetic Mg/Fe layered double hydroxide (magnetic LDH) nanocomposite was successfully pre- pared and used as a suitable adsorbent to simultaneously extract vitamins B1 and B6 from com- plex infant formula and food supplements. The important parameters affecting the extraction were optimized and the final parameters are as follows; the amount of adsorbent (20 mg), extrac- tion time (6 min), elution time (8 min), and elution solvent ethanol in 750 mL. As-prepared nanoad- sorbent was successfully in magnetic solid phase extraction set-up with no hazardous solvents and extraction performed in 6 min. The structure of nanoadsorbnet is lamellar, providing a wide surface to extract the analytes. The quantification of the analytes was accomplished using the liquid chromatography-tandem mass (LC/MS-MS) technique. Under optimum extraction conditions, the linearity ranged from 4 to 1000 ng/mL (vitamin B1) and 20 to 1000 ng/mL (vitamin B6), and the correlation of coefficients (R2 ) was obtained better than 0.99. The intra-day (n 1⁄4 3) and inter- day precisions (n 1⁄4 3 working days) calculated in the form of percent relative standard deviations (%RSDs) were obtained below 10.20%. The proposed method was successfully practiced for ana- lyzing vitamins B1 and B6 in several brands of infant formulas and food supplements.