مشخصات پژوهش

In this study, the osteo-inductive potential of Prunus amygdalus dulcis (SA) extract that loaded with different concentrations in poly-ε-Caprolactone/ gelatin (PCL/GEL) nanofibrous scaffolds was investigated. The morphology and composition of nanofibers were evaluated using field emission scanning electron microscope and FT-IR spectroscopy. Mechanical properties, hydrophilicity and pore structures were determined by tensile test, contact angle, and N2 adsorption–desorption isotherm, respectively. By incorporation of SA extract into PCL/GEL nanofibers, mechanical capabilities as well as their physical qualities were improved. The response of human dental pulp stem cells on prepared scaffolds were studied using MTT assay, alkaline phosphatase activity, alizarin red assay, and quantitave real-time PCR analysis. The SA loaded nanofibers presented excellent cell attachment and enhanced osteoblast proliferation and mineralization compared to the pristine PCL/GEL nanofibers. The analysis revealed the addition SA extract to PCL/GEL nanofibers increased the expression of osteogenic genes significantly. According to our findings, PCL/GEL/SA nanofibers show a lot of potential for bone tissue engineering.