مشخصات پژوهش

Entomopathogenic fungi produce extracellular enzymes to facilitate host infection, and these can also reduce metal ions to produce nanoparticles. In the present study, three isolates of Beauveria bassiana (JS1, JS2 and KA75) and one isolate of Metarhizium anisopliae (TT1) were evaluated for their ability to biosynthesize silver nanoparticles (AgNPs). In general, the best yields and smallest NP sizes were obtained at 60 ◦C and pH 7.0. Nanoparticle properties were studied using UV–visible spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy. Biosynthesized AgNPs ranged from 23 to 101 nm across, the smallest being produced by KA75, and the largest by TT1. UV–visible spectroscopy confirmed peak absorption of AgNPs in the range of 420–454 nm. AgNP antibacterial activity was highest against the gram-negative bacteria Pectobacterium carotovorum and Erwinia amylovora, and lower against the gram-positive Bacillus sp. AF1. JS1-AgNPs caused the greatest growth restriction of P. carotovorum at a concentration of 75 µL/mL at lower OD600 (0.25). Smaller AgNPs generally had better antifungal activities against B. bassiana, M. anisopliae, and the plant-pathogenic Rhizoctonia solani. Complete inhibition of vegetative growth of the JS2 fungus was obtained with TT1- synthesized AgNPs at 15 µL/mL, a control level similar to half the field rate of benomyl. Generally, fungal sporulation was more inhibited than vegetative growth, and all AgNPs showed good compatibility with the fungi at low concentrations. We conclude that AgNPs mycosynthesized by these entomopathogens have promising antibacterial and antifungal properties with potential for various applications.