مشخصات پژوهش

Soil salinity is one of the most important limiting reasons of crop production in arid and semi arid regions of Iran. The aim of this study was to investigate the possible amelioration of salt tolerance in wheat crop by using plant probiotic bacteria. In late spring and early summer of years 2018, healthy plants from Gramineae family were collected from saline or moderately saline fields of Azarbaijan Shahid Madani University (37.814589, 45.929741). Roots with surrounded soil was removed from ground and transported to the laboratory in sterile plastic bags. Halotolerant rhizobacteria were isolated on Nutrient Agar plates supplemented with 3% NaCl and purified by standard methods. Resulted isolates were screened based on their effect on wheat seed germination under salt stress. Germination of seeds treated with 12 and 18 dS/m salinity was drastically affected even in bacterized seeds. Germination of treated seeds with strain R7 under 3 and 6 dS/m salinity was significantly higher than those of other treatments. The effect of this bacterium on yield and morphophysiologic properties of wheat under salt stress was investigated in hydroponic system in greenhouse condition. Results showed that treated plants with this strain have higher level of chlorophyll b, proline, soluble sugars and leaf relative water content. Moreover, the height, dry mass of plants, number of seed per plant and yield per plant was higher in plants treated with this bacterium in comparison to control plants. Based on molecular and biochemical characterization this strain was identified as Serratia odorifera.